Released on: June 19, 2008, 1:18 am

Press Release Author: The Scott Partnership

Industry:

Press Release Summary: SANTA BARBARA, CALIFORNIA (June 18, 2008) - Wyatt Technology,
the world
leader in absolute macromolecular characterization products, announces the
publication of a new application note demonstrating the capability of its
DAWN Multi-Angle Light Scattering (MALS) and Optilab rEX (refractive index
detector) to achieve rapid, automated molecular characterization via a Zimm
plot experiment. Coupled to a binary HPLC pump and Wyatt\'s powerful ASTRA
software, the DAWN and Optilab rEX provided accurate light scattering data
and expedited results.

Press Release Body: NEW APPLICATION NOTE DEMONSTRATES WYATT TECHNOLOGY\'S TECHNOLOGY
CAPABILITY
TO PROVIDE RAPID, AUTOMATED MOLECULAR CHARACTERIZATION FOR ZIMM PLOT
EXPERIMENTS

SANTA BARBARA, CALIFORNIA (June 18, 2008) - Wyatt Technology, the world
leader in absolute macromolecular characterization products, announces the
publication of a new application note demonstrating the capability of its
DAWN Multi-Angle Light Scattering (MALS) and Optilab rEX (refractive index
detector) to achieve rapid, automated molecular characterization via a Zimm
plot experiment. Coupled to a binary HPLC pump and Wyatt\'s powerful ASTRA
software, the DAWN and Optilab rEX provided accurate light scattering data
and expedited results.

Historically, a syringe pump was used to prepare, mix and sequentially
inject five or more sample concentrations required for a standard Zimm plot.
The solutions had to be prepared prior to the experiment and entered by
hand. Overall, this was an intensive and time-consuming procedure.
Additionally, air was often inadvertently injected into the detectors,
resulting in large noise spikes in the data. A binary HPLC pump was used in
conjunction with the Optilab to automatically determine the concentrations
during the experiment. The differential refractive index values
corresponding to the concentrations of the solutions are collected by ASTRA,
eliminating the need to determine the concentrations a priori.

For this particular application, a 545kDa Dextran natural polymer was
dissolved at 2.5 mg/mL in buffer and placed in a reservoir, whereas buffer
alone was placed in a separate reservoir. A low volume 0.45 mm filter was
installed between the binary HPLC pump and a DAWN HELEOS multi angle light
scattering detector (Wyatt). The Optilab rEX was installed downstream of the
light scattering detector. The flow rate was set to 1 mL/min and the pump
was set to deliver a concentration series of 0% A to 100% A and then back to
0% A. Each concentration was run for three minutes for a total of 3 mL per
step. This volume ensured that a plateau was attained in both the static
light scattering and the concentration detector. With plateauing, identical
concentrations were present in both detectors.

The results of the experiment clearly demonstrated that automated sample
dispensing and concentration detection ensure clean data acquisition,
enabling accurate detection of the molar mass, size and second virial
coefficient in fewer than 30 minutes. Data quality was found to exceed that
of standard syringe pump runs.

For more information on Wyatt Technology\'s Optilab rEX refractive index
detector and ASTRA software or to obtain a copy of this application note,
please visit www.wyatt.com or e-mail info@wyatt.com

###
About Wyatt
Based in Santa Barbara, California, Wyatt Technology is the world\'s leading
provider of instruments for absolute macromolecular characterization. With
over 40 years\' experience developing multi-angle light scattering detectors,
working with customers in the biotechnology, chemical, petrochemical,
pharmaceutical, academic and government arenas, Wyatt prides itself on its
entrepreneurial spirit, and the uniqueness of its offerings. The Company\'s
groundbreaking technology and uncompromising levels of customer care make
Wyatt the global hallmark in its field. For more information, please visit
www.wyatt.com

For further press information please contact: Laura Browne, The Scott
Partnership, The Old Barn, Holly House Estate, CRANAGE, Middlewich, Cheshire
CW10 9LT, United Kingdom Tel: + 44 1606 837787 Fax: +44 1606 837757 mail
to:pr@scottmail.co.uk


Web Site: http://

Contact Details: Laura Browne, The Scott
Partnership, The Old Barn, Holly House Estate, CRANAGE, Middlewich, Cheshire
CW10 9LT, United Kingdom Tel: + 44 1606 837787 Fax: +44 1606 837757 mail
to:pr@scottmail.co.uk